Business Coach and Founder of Clutch Consulting, Nathan R Mitchell

Posted by : partner | On : June 2, 2012

Los Angeles, CA (PRWEB) April 18, 2012

Clutch Consulting, a business consulting and coaching company dedicated to Growing Businesses and Empowering People, announces the launch of an aggressive affiliate program in an effort to help change more businesses and more lives.

Trusted Business Advisor, Nathan R Mitchell, announces this on the heels of bringing The Winning Mindset Series, to market. The Winning Mindset Series is a monthly subscription-based membership program designed to help existing business owners and aspiring entrepreneurs acquire the knowledge, tools, and resources they need to succeed.

Many businesses dont understand the importance of joint ventures and affiliate marketing, says Mitchell. Affiliate programs and joint ventures are absolutely necessary in this economy if businesses are looking to extend their market reach.

And thats exactly what Mitchell and his company, Clutch Consulting, are looking to do.

I want to help as many people as possible achieve their goals and dreams in the wonderful world of free enterprise, says Mitchell. Our monthly subscription-based membership program The Winning Mindset Series is one of the ways we are looking to do that moving forward.

The good news is Mitchell and Clutch Consulting have put together an aggressive affiliate program to help spread the word and compensate others in the process.

We are looking to partner with people whose mission is in alignment with our own organizational vision and objectives, says Mitchell. Obviously, we wont work with just anyone. Our affiliate program requires an application and approval process, but for those we do accept into the program, its a great opportunity to create a long-term mutually beneficial relationship.

Subscribers to The Winning Mindset Series receive

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It seems that there will be an announcement at VanaFest related to Nyzul Isle Assault. But what could it be? Head over the the official forums and join the discussion!

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Seven dead, 30 wounded in north Lebanon clashes

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Training cells to perform Boolean functions? It's logical

Thursday, May 31, 2012

Johns Hopkins scientists have engineered cells that behave like AND and OR Boolean logic gates, producing an output based on one or more unique inputs. This feat, published in the May issue of Nature Chemical Biology, could eventually help researchers create computers that use cells as tiny circuits.

Study leader Takanari Inoue, Ph.D., an assistant professor in the Department of Cell Biology and member of the Institute of Basic Biomedical Sciences' Center for Cell Dynamics at the Johns Hopkins University School of Medicine, explains that many researchers are striving to mimic devices in everyday use by engineering new qualities into biological materials, including biomolecules and cells. Several of those engaged in this relatively new field, known as synthetic biology, have tried to create biological computers.

At the heart of both the biological and the more everyday silicon-based variety of computers are Boolean logic gates, which produce responses that vary depending on what type and how many inputs they receive. For example, AND gates need two unique inputs to generate an output. In contrast, OR gates generate an output based on whether they receive one input, or another, or both.

Inoue says that previous research has shown some success in generating logic gates based on biomolecules in test tubes or petri dishes. However, he adds, developing logic gates using whole cells has proven significantly trickier. Most previous efforts have taken advantage of cells' transcriptional machinery ? the cellular processes that read genes to create proteins ? to generate an output signal. But transcription can be a slow process, taking from minutes to days to produce the desired response.

"People like to have speedy computation," Inoue says. "We were hoping to achieve computation in cells on the order of seconds, which is significantly faster than what people have achieved thus far."

To accomplish their goal, the researchers used a technique called chemically inducible dimerization, or CID. This tool takes advantage of natural biological mechanisms that bring together two proteins into a complex in the presence of a chemical.

Since AND and OR gates generate a response based on two different inputs, either together or separately, the researchers needed two different CID systems that didn't compete or overlap with each other. They relied on one system that's been studied for years, which brings two proteins, called FRB and FKBP, together in the presence of a drug called rapamycin. Rapamycin comes from bacteria, and FRB and FKBP come from animals.

In addition, they used a second CID system that brings together two other proteins, known as GID1 and GAI, in the presence of a plant hormone called gibberellin. Since this system is native to plants, the gibberellin-based system doesn't compete with the rapamycin-based one, Inoue explains.

The researchers engineered mammalian cells that produce all four of the requisite proteins, as well as a response when the right two proteins came together. When either FRB and FKBP or GID1 and GAI linked up, the cell's membrane developed ruffles easily visible under a microscope.

To create the OR gate, FRB and GAI were bound together at the cell membrane, while FKBP and GID1 were bound together floating freely in the cell. Adding either rapamycin, gibberellin, or both to cells brought the freely floating complex to the one at the cellular membrane, linking up the matching proteins and triggering the output signal.

To create the AND gate, the researchers placed just GAI at the cell membrane, with just FRB and complexes of FKBP and GID1 free-floating in the cell. This system required all four proteins to link up to produce membrane ruffling, which wouldn't occur without both input chemicals.

Tests showed that each of the engineered cellular logic gates produced the desired response reliably, in a matter of seconds. Additionally, as a second proof of principle, the researchers generated similar logic gates that used fluorescence as an output, which worked just as well and quickly.

Eventually, Inoue notes, researchers might use similar cellular logic gates to build larger, more complex circuits that could form the basis for computers that use cells as basic units. In the meantime, these individual cellular circuits could be engineered to produce specific outputs in the presence of chemicals, making them useful detectors or diagnostic agents. He adds that researchers might also use synthetic logic gate systems like this one to study how cells naturally produce outputs to keep bodily functions running smoothly.

###

Johns Hopkins Medical Institutions: http://www.hopkinsmedicine.org

Thanks to Johns Hopkins Medical Institutions for this article.

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VIA ARTiGO A1200 lets you roll your own miniscule, fanless PC

Most ultra-compact, fanless PCs are intended for the embedded market -- that is, not for the proletariat to tinker with directly. VIA's no stranger to serving that crowd, but it's making a point of addressing home users who want this class of PC for DIY projects, like home media servers, with the ARTiGO A1200. The new design is still using the dual-core, 1GHz Eden X2 of VIA's usual embedded PCs at its heart, but it comes wrapped in a smaller, more eye-catching package with HDMI video out and support for Windows 7. That's not to say that the A1200 represents a softening, inside or out. The PC can still survive temperatures between 32F and 113F with dust resistance in the bargain, and there's an array of ports you're still less likely to find on an everyday computer, such as a CFast slot (for storage) and dual gigabit Ethernet jacks. The $320 it costs to buy the new ARTiGO today won't get you an OS, RAM or a hard drive, but its ability to survive for years in a hot, dusty closet just might be worth the cost.

Continue reading VIA ARTiGO A1200 lets you roll your own miniscule, fanless PC

VIA ARTiGO A1200 lets you roll your own miniscule, fanless PC originally appeared on Engadget on Thu, 31 May 2012 09:42:00 EDT. Please see our terms for use of feeds.

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Get Radar Detectors ? Blog Archive ? Social Marketing

16:36 Social Media social marketing The Facebook IPO has failed even in the social network because nobody talks about it: the conversation is on Twitter. In spite of much talk of social networks and users, not much data about the real situation of women and their full extent. The Cocktail Analysis IV presented the wave of social networking Observatory. In this study we can observe several points, the general trend of users in the use of social networks since its inception to the present. 91% of Internet users have an active account and the average is 2.3 accounts. In the field of mobile, 55% of Internet users access this device from the same social networks. Plasma is also how social networks are an established reality, and that 92% of users leave a social network that is maintained in others. Due to the proliferation of platforms more and more offers and more options. So, the question is where are the Internet. Facebook has had a slight increase, reaching 85%. But more importantly, has become universal, with some users of 30.5 years on average. Tuenti is shown standing, with no growth, with younger users (25.5 years on average). Regarding Twitter, is booming, its use is increasing and has doubled its penetration from the previous study with 32%. As for the performance of brands in social networks, although the comments on the same purchase aroused the interest of consumers, are not priority areas to search for information on products. Social networks should not overshadow the other channels, as the websites and search engines are presented as the most important spaces for information when there is intent to purchase. The official website is still the option that most weight, being also important the search engines, forums and blogs in the case of mobile operators and technology, and less fashionable. A new bottle of red summer There are many brands for which the results they get from their online presence is clearly inadequate, more than 84% according to a report by BaseKit. Of all the brands with corporate portals, 1 in 6 is satisfied and 25% think your competition delivers better quality. For 75% of SMEs as com online presence, the website is still synonymous with influence and trust, although the data show 11% of companies whose Web site is detrimental to their reputation online. It is obvious from reading the report, SMEs feel somewhat at a disadvantage compared to the younger generation, seeking a road map for quality not found, not in vain for a 7% disagreement reflects the "technical assistance" and need a way to delve into a universe that requires resources ? but do not have them, which means that the chip change imposed on us by the penetration of social media in our lives, has not yet completed and is ? adapt our rhythms dizzy appearance of new technologies, tools and applications, we are not proving easy. We will be the first digital generation in history ? digital dinosaurs as the study says, that beyond an analysis of the obstacles faced by SMEs, should be taken as an important source of information for professionals focused on providing services to small and medium enterprises. SMEs know they are losing opportunities to stay out of the mobile Web and Social Media but still are very lost in the new way to build a business and grow from the emotions. I leave the report ? gives me lots of information to identify needs of others, is in them that we need to focus not it? Tell me what you think. We strive to properly manage our social networks. We are looking for or create interesting content. We interact with our fans, sparking conversations, answering their questions and complaints. Create contests, we ask questions We have already said once that social media equal opportunities for promotion of large multinational companies and small and medium enterprises. Democratize marketing and make it more accessible, both from the economic standpoint, and from the coach. In fact, some experts go further and suggest that small businesses can take much more than large social networks. Small business, personal brands and bloggers can benefit most from social media. On the Web Social Mouths, Francisco Rosales explains the reasons why small businesses have an advantage over large when using social media as marketing tool. This, despite the huge budgets and unlimited resources at their disposal the latter. For a marketing campaign in social media to succeed you need a marketing strategy. In this sense, small firms have an advantage: flexibility. It is necessary to develop an endless document explaining the plan of that strategy. Is sufficient (but on the other hand, it is essential, lest we forget) have clear objectives and be familiar with the area in which the activity occurs in the company. From there, you can make changes on the fly, something unthinkable for large multinationals. In big companies, bureaucracy can be endless: documents to make every little decision, inform many employees, sometimes in different countries, with an appropriate tone

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